========================== Tissue-specific Expression Analysis ========================== Currently, the tissue-specific patterns analysis provide valuable information about their potential functions for further studies. Read before use ------------------ Users need to prepare two files, gene expression matrix and metadata information. **The gene expression matrix:** The first column is Geneid, other columns are data. The name of first column must be Geneid. Data from excel (format: xlsx). **The metadata:** The first column is sample names, the second column is treatment conditions, and the third column is group names. Colnames must be Samples, Names, and Groups. Data from excel (format: xlsx). **Note:** The order of the sample names in both files must be consistent. tau value calculator ------------------ This tool provides a tau value calculator. The tau values varies from 0 to 1. Gene with tau value close to 1 represented tissue specificity of gene expression, while gene with tau value close to 0 indicated sharing of gene expression across different tissues. **Input example data:** `Gene expression matrix `_, `Metadata information `_. .. image:: ../../_static/images/tvalue.png :width: 100% :align: center Venn plot ------------------ This tool provides vennplot for tissue-specific gene expression. Tissue-specific genes are a class of genes whose functions and expressions are preferred in one or several tissues restrictedly. Identification of tissue-specific genes is essential for discovering multi-cellular biological processes such as tissue-specific molecular regulations, tissue development, physiology, and the pathogenesis of tissue-associated diseases. Users need to prepare two files, gene expression matrix and metadata information. **The gene expression matrix:** The first column is Geneid, other columns are data. The name of first column must be Geneid. Data from excel (format: xlsx). **The metadata:** The first column is sample names, the second column is treatment conditions, and the third column is group names. Colnames must be Samples, Names, and Groups. Data from excel (format: xlsx). **Note:** The order of the sample names in both files must be consistent. **Input example data:** `Gene expression matrix `_, `Metadata information `_. .. image:: ../../_static/images/venn.png :width: 100% :align: center Bubble Plot ------------------ This tool provides a bubble plot, which shows gene expression specificity (indicated by dot size and color) among tissues. Users need to prepare three files, gene expression matrix, metadata information, and genes list. **The gene expression matrix:** The first column is Geneid, other columns are data. The name of first column must be Geneid. Data from excel (format: xlsx). **The metadata:** The first column is sample names, the second column is treatment conditions, and the third column is group names. Colnames must be Samples, Names, and Groups. Data from excel (format: xlsx). **The gene list:** The first column is Geneid. The name of first column must be Geneid. Data from excel (format: xlsx). **Note:** The order of the sample names in both gene expression matrix and metadata information files must be consistent. **Input example data:** `Gene expression matrix `_, `Metadata information `_, `Gene list `_. .. image:: ../../_static/images/bubble.png :width: 100% :align: center